CGSC Strain#: 5162
Strain Designation: D21f2      Source of Strain: H.G. Boman
Sex: F- Chromosomal Markers: proA23, lac-28, tsx-81, trp-30, his-51, rpsL173(strR), rfa-1, rfa-31, ampCp-1
Strain Comments:

• ampCp-1-- is a promoter mutation; AmpC⁺ strains carrying this allele produce about 10X the amount of beta lactamase found in wild-type strains. In absence of other amp alleles, use 10-15 ug/ml Amp.
• ampCp-1 was formerly called ampA1
• rfa-1 was formerly called lpsA1
• rfa-31 was formerly called lpsD31
• strR = streptomycin resist.

References:

• Boman, HG, K Nordstrom, S Normark 1974. Penicillin resistance in Escherichia coli K12: synergism between penicillinases and a barrier in the outer part of the envelope. Ann. N. Y. Acad. Sci. 235(0):569-86.
• Havekes, L, J Tommassen, W Hoekstra, B Lugtenberg 1977. Isolation and characterization of Escherichia coli K-12 F- mutants defective in conjugation with an I-type donor. J. Bacteriol. 129(1):1-8.