CGSC Strain#: 5194
Strain Designation: NO864     
Source of Strain: Nomura & Engbaek
Sex: Hfr
Point of Origin#: 12
Map Position: 68.80
Direction of Marker Transfer: Clockwise
Chromosomal Markers: fhuA2::IS2,
lacY1,
tsx-1,
glnX44(AS)
,
gal-6,
λ-,
purF1,
rpsE116(SpcR)
,
rplD1,
fusA1,
rpsL117(strR)
,
xyl-7,
mtlA2,
metB1Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- purF1-- is a very stable mutation and may be an intragenic deletion.
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rplD1 was formerly called eryC-1
- xyl-7 was formerly called xylA7
- ::IS2 = Insertion of IS2
- AS = amber(UAG) suppressor
- SpcR = spectinomycin resistant
- strR = streptomycin resist.
References: - Nomura, M, F Engbaek 1972. Expression of ribosomal protein genes as analyzed by bacteriophage Mu-induced mutations. Proc. Natl. Acad. Sci. U.S.A. 69(6):1526-30.