CGSC Strain#: 5608
Strain Designation: WA704      Source of Strain: W. Arber
Sex: F- Chromosomal Markers: lacY1 or Δ(cod-lacI)6, glnX44(AS), galK2(Oc), galT22, λ^-, e14-, rfbC1, metB1, mcrB1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation

References:

• Wood, W.B. 1966. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA. J.Mol.Biol. 16:118-133