CGSC Strain#: 5615
Strain Designation: WA960      Source of Strain: W.B. Wood
Sex: F- Chromosomal Markers: leuB6(Am), fhuA21, lacY1 or Δ(cod-lacI)6, glnX44(AS), λ^-, metB1, thiE1, [r+B,m+B]
Strain Comments:

• The strain is r+B,m+B, a K-12 B/r hybrid, K-12 with B modification and restriction.
• This strain is an E. coli K-12 derivative which has received the segment hsd-thr from E. coli B. The strain is r⁺_B, m⁺_B.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• [r+B,m+B]-- is a K-12 and B/r hybrid. K-12 with B restriction and modification.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• Wood, W.B. 1966. Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA. J.Mol.Biol. 16:118-133