CGSC Strain#: 5625
Strain Designation: WM301      Source of Strain: W. Messer
Sex: F- Chromosomal Markers: leuB19, lac-11, sulA1, trp-25, thyA59, deoB23, arg-28, [r+B,m+B], pro-19, str-56, his-47, met-55, gal-11
Strain Comments:

• The strain is r⁺_B,m⁺_B.
• thyA59-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• arg-28-- In cgsc: AC2519 F+. Source: Boyer E.coli B/r.
• [r+B,m+B]-- is a K-12 and B/r hybrid. K-12 with B restriction and modification.

References:

• Beyersmann, D., W. Messer, M. Schlicht 1974. Mutants of Escherichia coli B/r defective in deoxyribonucleic acid initiation: dnaI, a new gene for replication. J.Bacteriol. 118:783-789