CGSC Strain#: 4292
Strain Designation: KL712     
Source of Strain: M.C. Cappello
Other Designations: KLF43-A
Sex: F'
Episome/Plasmid: F143-1
Plasmid Markers/Mutations: relA1,
recA1 Chromosomal Markers: galK35,
λ-,
pyrD34,
e14-,
trpC45,
his-68,
tyrA2,
recA1,
thyA33,
rpsL118(strR)
,
malT1(λ
R)
,
xyl-7,
mtlA2,
thiE1Strain Comments: - This strain must be grown on medium containing thi, his, try, ura and lacking tyr, thy. F143-1 carries the relA1 mutation.
- galK35-- is Brenner's gal-U99 from Hfr 3000 U99 and has been called galK99
- e14--- : Loss of the e14 element makes the strain McrA-.
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- thyA33-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- LamR = Lambda-resistant
- strR = streptomycin resist.
References: