CGSC Strain#: 4301
Strain Designation: KL716     
Source of Strain: M.C. Cappello
Other Designations: KLF48/KL158
Sex: F'
Chromosomal Markers: Δ(gpt-proA)62,
lacY1,
tsx-29,
glnX44(AS)
,
galK2(Oc)
,
λ-,
aroD5,
hisG4(Oc)
,
recA1,
xylA5,
argE3(Oc)
,
thiE1,
str-148Strain Comments: - This strain must be grown on medium containing B1, Pro, Arg, and lacking His, Try, Tyr, Phen.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: