CGSC Strain#: 6753
Strain Designation: BW5660     
Source of Strain: B.L. Wanner
Sex: Hfr
Point of Origin#: 66(ColV)
Map Position: 43.80
Direction of Marker Transfer: Clockwise
Chromosomal Markers: Δ(gpt-lac)5,
glnX44(AS)
,
λ-,
srlD300::Tn10,
relA1,
spoT1,
thiE1Strain Comments: - Δ(gpt-lac)5-- is the deletion Δ( pro-lac)X111 of Jacob and originated in the strain Hfr 3000 X111.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- srlD300::Tn10-- : The insertion was determined by sequencing to be within srlD (F.W.Pons, pers.commun., Oct.2000)
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- srlD300::Tn10 was formerly called srlC300::Tn10
- thiE1 was formerly called thi-1
- = tetracycline resistant
- AS = amber(UAG) suppressor
References: - Wanner, B.L. 1986. Novel regulatory mutants of the phosphate regulon in Escherichia coli K-12. J.Mol.Biol. 191:39-58