CGSC Strain#: 6520
Strain Designation: NS4      Source of Strain: M. Matsuhashi
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), secA206(aziR), fhuA61, lacY1, galT23, λ^-, trp-84, his-215, thyA710, lpcB4, rpsL263(strR), xylA5, mtl-1, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• galT23-- : This is the standard gal₆ of Morse & Lederberg.
• thyA710-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• aziR = azide resistance
• strR = streptomycin resist.

References:

• Tamaki, S., T. Sato, M. Matsuhashi 1971. Role of lipopolysaccharides in antibiotic resistance and bacteriophage adsorption of Escherichia coli K-12. J.Bacteriol. 105:968-975