CGSC Strain#: 6689
Strain Designation: JF136      Source of Strain: J.O. Falkinham
Sex: Hfr    Point of Origin#: 12   Map Position: 68.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: glnX44(AS), λ^-, purF1, xyl-7, avtA1, ΔargH1
Strain Comments:

• JF136 was constructed by transducing strain AB1931 to Met⁺ with P1 grown on strain JF25.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• purF1-- is a very stable mutation and may be an intragenic deletion.
• avtA1-- : In the absence of an ilvE (transaminase B) mutation, avtA^- causes no nutritional requirement
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• xyl-7 was formerly called xylA7
• AS = amber(UAG) suppressor

References:

• Falkinham, J.O. 1979. Identification of a mutation affecting an alanine-alpha-ketoisovalerate transaminase activity in Escherichia coli K-12. Mol.Gen.Genet. 176:147-149