CGSC Strain#: 6706
Strain Designation: MV1138      Source of Strain: M.R. Volkert
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ^-, Rac-0, hisG4(Oc), rfbC1, [recAo-281](Const), srlD300::Tn10, rpsL31(strR), kdgK51, xylA5, rfa-300, mtl-1, argE3(Oc), thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• [recAo-281](Const)-- was transduced in from E. coli B.
• srlD300::Tn10-- : The insertion was determined by sequencing to be within srlD (F.W.Pons, pers.commun., Oct.2000)
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• [recAo-281](Const) was formerly called rnmB281
• rfbC1 was formerly called rfbD1
• srlD300::Tn10 was formerly called srlC300::Tn10
• thiE1 was formerly called thi-1
• = tetracycline resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Const = constitutive
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Volkert, M.R., D.M. Spencer, A.J. Clark 1979. Indirect and intragenic suppression of the lexA102 mutation in E. coli B/r. Mol.Gen.Genet. 177:129-137
• Volkert, M.R., L.J. Margossian, A.J. Clark 1981. Evidence that rnmB is the operator of the Escherichia coli recA gene. Proc.Natl.Acad.Sci.USA 78:1786-1790
• Uhlin, B.E., M.R. Volkert, A.J. Clark, A. Sancar, W.D. Rupp 1982. Nucleotide sequence of a recA operator mutation. Mol.Gen.Genet. 185:251-0
• Clark, AJ, MR Volkert, LJ Margossian, H Nagaishi 1982. Effects of a recA operator mutation on mutant phenotypes conferred by lexA and recF mutations. Mutat. Res. 106(1):11-26.