CGSC Strain#: 6712
Strain Designation: DJK455      Source of Strain: D. Kolodrubetz
Sex: F- Chromosomal Markers: araD591, araCc-1336(Const), Δ(codB-lacI)3, λ^-, ara(FG)63, gyrA270(NalR), relA1, thyA750, araE455, spoT1, thiE1
Strain Comments:

• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• thyA750-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• thiE1 was formerly called thi-1
• Const = constitutive
• NalR = Nalidix.acidResist.

References:

• Kolodrubetz, D. 1980. Thesis, Brandeis University 0:26-29