CGSC Strain#: 6716
Strain Designation: KLC1284     
Source of Strain: J.W. Chase
Sex: Hfr
Point of Origin#: 1
Map Position: 96.80
Direction of Marker Transfer: Clockwise
Chromosomal Markers: lacZ125(Am)
,
λ-,
e14-,
tyrT66(AS)
,
trpC83::Tn10,
relA1,
spoT1Strain Comments: - lacZ125(Am)-- is the amber mutation from Zipser's strain NG125 and Brenner's CA274. It was formerly called lac125am by Brenner. In `interval 6' of Newton et al. JMB 14:290 (1965).
- e14--- : Loss of the e14 element makes the strain McrA-.
- trpC83::Tn10-- PCR analysis by Nichols et al. 1998 placed this insertion in the trpC rather than trpB gene (at nucleotide 1,317,596 or 28.401 min.)
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- trpC83::Tn10 was formerly called trpB83::Tn10
- tyrT66(AS) was formerly called supF66
- tyrT66(AS) was formerly called su+III
- = tetracycline resistant
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
References: