CGSC Strain#: 6728
Strain Designation: DF920     
Source of Strain: D. Fraenkel
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
ompF627(T2R)
,
pfkB20::Tn10,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
rrnB-2,
mcrB1,
creC510Strain Comments: - pfkB20::Tn10-- The Tn 10 insertion was erroneously referred to as pkfB21::Tn 10 in Tabl -- e 1 of Daldal and Fraenkel 1981.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- = tetracycline resistant
- Const = constitutive
- T2R = T2 Resistant
References: - Daldal, F., D. Fraenkel 1981. Tn10 insertions in the pfkB region of Escherichia coli. J.Bacteriol. 147:935-943
- Daldal, F., J. Babul, V. Guixe, D. Fraenkel 1982. An alteration in phosphofructokinase 2 of Escherichia coli which impairs gluconeogenic growth and improves growth on sugars. Eur.J.Biochem. 126:373-379