CGSC Strain#: 6737
Strain Designation: ZIP2286B     
Source of Strain: D. Zipser
Other Designations: CSH1
Sex: F-
Chromosomal Markers: lacZ2286(Am)
,
λ-,
trpA49(Am)
,
relA1,
rpsL150(strR),
spoT1Strain Comments: - lacZ2286(Am)-- is a UAG mutation in the first segment of the lacZ gene (Zipser et al., 1970).
- trpA49(Am)-- is the amber mutation formerly called trp-8am by Brenner.
- rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- Am = amber(UAG) mutation
- strR = streptomycin resist.
References: - Newton, W.A., J.R. Beckwith, D. Zipser, S. Brenner 1965. Nonsense mutants and polarity in the lac operon of Escherichia coli. J.Mol.Biol. 14:290-296
- Zipser, D, S Zabell, J Rothman, T Grodzicker, M Wenk 1970. Fine structure of the gradient of polarity in the z gene of the lac operon of Escherichia coli. J. Mol. Biol. 49(1):251-4.