CGSC Strain#: 6768
Strain Designation: SC122     
Source of Strain: S. Cooper
Sex: F-
Chromosomal Markers: lacZ53(Am)
,
phoA5(Am)
,
λ-,
tyrT47(OS)
,
tyrT91(ts,AS)
,
trp-48(Am)
,
relA1,
rpsL150(strR),
malT66(Am,λ
R)
,
spoT1Strain Comments: - Other markers which might be in this strain: tonA22, ompF627(T2R), phoU35, bglCop16.
- lacZ53(Am)-- is the Paris lac-Y14 amber.
- phoA5(Am)-- is Garen's phoAH12.
- rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- tyrT91(ts,AS) was formerly called supC91
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- OS = ochre(UAA) suppressor
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Cooper, S, T Ruettinger 1975. A temperature sensitive nonsense mutation affecting the synthesis of a major protein of Escherichia coli K12. Mol. Gen. Genet. 139(2):167-76.
- Neidhardt, F.C., R.A. VanBogelen 1981. Positive regulatory gene for temperature-controlled proteins in Escherichia coli. Biochem.Biophys.Res.Commun. 100:894-900