CGSC Strain#: 6792
Strain Designation: DF949     
Source of Strain: D. Fraenkel
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
ompF627(T2R)
,
zdi-925::Tn10,
gnd-1,
relA1,
pitA10,
spoT1Strain Comments: - zdi-925::Tn10-- cotransduces ca. 65% with pps and 55% with pfkB. Found by PCR (Nichols et al. 1998) to be an insertion at nucleotide 1,776,386 (38.291 min.)
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- = tetracycline resistant
- T2R = T2 Resistant
References: - Daldal, F., J. Babul, V. Guixe, D. Fraenkel 1982. An alteration in phosphofructokinase 2 of Escherichia coli which impairs gluconeogenic growth and improves growth on sugars. Eur.J.Biochem. 126:373-379