CGSC Strain#: 6794
Strain Designation: UNC1085      Source of Strain: A. Sancar
Sex: F- Chromosomal Markers: pro-82, glnX44(AS), phr-1, ΔnadA71, λ^-, Δ(recA-srl)306, relA1, endA1, rpsL286, thiE1, uvrA281, hsdR17
Strain Comments:

• This strain can be used with the Maxicell technique. Unlike the original Maxicell strain (CSR603), it is hsdR^-, endA^-.
• pro-82-- is in proA or proB.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• Δ(recA-srl)306-- removes all, or nearly all, of the recA gene and extends into srlR, but does not remove the Tn10 insertion in srlR
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• AS = amber(UAG) suppressor

References:

• Sancar, A, AM Hack, WD Rupp 1979. Simple method for identification of plasmid-coded proteins. J. Bacteriol. 137(1):692-3.