CGSC Strain#: 6848
Strain Designation: AX621      Source of Strain: J.R. Walker
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), ftsA1882(ts), Δ(gpt-proA)62, lacY1, tsx-33, qsr'-0, glnX44(AS), galK2(Oc), λ^-, Rac-0, hisG4(Oc), rfbC1, rpsL31(strR), kdgK51, xylA5, mtl-1, argE3(Oc), thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.
• ts = Temperature Sensitive

References:

• Allen, JS, CC Filip, RA Gustafson, RG Allen, JR Walker 1974. Regulation of bacterial cell division: genetic and phenotypic analysis of temperature-sensitive, multinucleate, filament-forming mutants of Escherichia. J. Bacteriol. 117(3):978-86.
• Walker, JR, A Kovarik, JS Allen, RA Gustafson 1975. Regulation of bacterial cell division: temperature-sensitive mutants of Escherichia coli that are defective in septum formation. J. Bacteriol. 123(2):693-703.
• Fletcher, G, CA Irwin, JM Henson, C Fillingim, MM Malone, JR Walker 1978. Identification of the Escherichia coli cell division gene sep and organization of the cell division-cell envelope genes in the sep-mur-ftsA-envA cluster as determined with specialized transducing lambda bacteriophages. J. Bacteriol. 133(1):91-100.