CGSC Strain#: 6969
Strain Designation: KNK402     
Source of Strain: K.N. Kreuzer
Sex: F+
Chromosomal Markers: tsx-79,
[gyrA43](ts)
,
thyA755,
endA2,
rpsL8 or rpsL154(strR)
,
polA1(Am)
Strain Comments: - [gyrA43](ts)-- The gyrA allele was transduced in from E. coli K-12.
- [gyrA43](ts)-- has a high spontaneous reversion frequency. We suggest frequent verification of the Ts phenotype.
- thyA755-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- polA1(Am)-- normal levels exonuc.;premature term.of polymerase <1% WT level. MMSS,UVS, viable at all temps. Grindley MGG143
- = good miniprep dsDNA quality
- Am = amber(UAG) mutation
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Kreuzer, KN, K McEntee, AP Geballe, NR Cozzarelli 1978. Lambda transducing phages for the nalA gene of Escherichia coli and conditional lethal nalA mutations. Mol. Gen. Genet. 167(2):129-37.