CGSC Strain#: 7059
Strain Designation: AE1010     
Source of Strain: P.M. Silverman
Sex: F-
Chromosomal Markers: leuB6(Am)
,
fhuA2::IS2,
lacY1,
glnX44(AS)
,
gal-6,
λ-,
cpxB11,
hisG1,
rfbC1,
thyA758,
galP63,
argG6,
rpsL104,
malT1(λ
R)
,
xyl-7,
mtlA2,
metB1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- cpxB11-- was formerly called cpxB1.
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- thyA758-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- xyl-7 was formerly called xylA7
- = strep-resistant
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
References: - McEwen, J., P. Silverman 1980. Genetic analysis of Escherichia coli K-12 chromosomal mutants defective in expression of F-plasmid functions: identification of genes cpxA and cpxB. J.Bacteriol. 144:60-67