CGSC Strain#: 7073
Strain Designation: A237      Source of Strain: Russel & Model
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, trxB20, zca-1230::Tn10, ompF627(T2R), serU132(AS), fadL701(T2R), relA1, pitA10, spoT1, mcrB1, creC510
Strain Comments:

• zca-1230::Tn10-- cotransduces 88% with aroA. PCR analysis (Nichols et al. 1998) places this insertion at nucleotide 946,252 (20.297 min.)
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• serU132(AS) was formerly called supD32
• serU132(AS) was formerly called Su1⁺
• zca-1230::Tn10 was formerly called zbj-1230::Tn10
• = tetracycline resistant
• AS = amber(UAG) suppressor
• Const = constitutive
• T2R = T2 Resistant

References:

• Russel, M., P. Model 1985. Direct cloning of the trxB gene that encodes thioredoxin reductase. J.Bacteriol. 163:238-242