CGSC Strain#: 4362
Strain Designation: KL129      Source of Strain: M.C. Cappello
Sex: F- Chromosomal Markers: leuB6(Am), fhuA2::IS2, lacY1, tsx-1, glnX44(AS), gal-6, λ^-, hns-1, rfbC1, recA1, thyA38, galP63, argG6, aroB11, malT1(λ^R), xyl-7, mtlA2, metB1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• thyA38-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• hns-1 was formerly called osmZ1
• hns-1 was formerly called cur-1
• rfbC1 was formerly called rfbD1
• xyl-7 was formerly called xylA7
• ::IS2 = Insertion of IS2
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant

References:

• Low, K.B. 1973. Rapid mapping of conditional and auxotrophic mutants of Escherichia coli K-12. J.Bacteriol. 113:798-812