CGSC Strain#: 4374
Strain Designation: KL403     
Source of Strain: M.C. Cappello
Sex: F-
Chromosomal Markers: araC14,
leuB6(Am)
,
lacZ36,
proC32,
λ-,
sup-78(Mal+)
,
hisF860(Stable)
,
thyA54,
rpsE2115(SpcR)
,
rpsL109(strR)
,
malA38,
xylA5,
mtl-1,
polA1(Am)
,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- sup-78(Mal+)-- is an uncharacterized mutation near his which confers a Mal+ phenotype.
- thyA54-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- malA38-- : In Χ65 a Mal+ phenotype may have resulted from suppression of malA1, but in the absence of evidence, we show the mutations as malA38 and sup-78.
- polA1(Am)-- normal levels exonuc.;premature term.of polymerase <1% WT level. MMSS,UVS, viable at all temps. Grindley MGG143
- proC32 was formerly called proC65
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- SpcR = spectinomycin resistant
- Stable = stable, non-reverting
- strR = streptomycin resist.
References: - Lloyd, R.G., K.B. Low, G.N. Godson, E.A. Birge 1974. Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive RecA- phenotype. J.Bacteriol. 120:407-415