CGSC Strain#: 4392
Strain Designation: S591      Source of Strain: R.G. Lloyd
Sex: F- Chromosomal Markers: araBAD-9, leu-86, fhuA1, Δ(gpt-proA)62, lacY1 or lacZ4, tsx-3, glnX44(AS), galK2(Oc), λ^-, trpE3(Oc), Rac-0, hisG4(Oc), rfbC1, rpsL8 or rplL9(L?), malT1(λ^R), mtl-1, ilvE12, polA591, thiE1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Lloyd, R.G., K.B. Low, G.N. Godson, E.A. Birge 1974. Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive RecA- phenotype. J.Bacteriol. 120:407-415
• Stacey, KA, RG Lloyd 1976. Isolation of rec- mutants from an F-prime merodiploid strain of Escherichia coli K-12. Mol. Gen. Genet. 143(2):223-32.