CGSC Strain#: 6607
Strain Designation: GU13     
Source of Strain: I.R. Beacham
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
Δ(gpt-proA)62,
lacY1,
tsx-33,
qsr'-0,
glnX44(AS)
,
galK2(Oc)
,
λ-,
hisG4(Oc)
,
upp-31,
rpsL31(strR)
,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1,
cpdB1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Beacham, I.R., R. Kahana, L. Levy, E. Yagil 1973. Mutants of Escherichia coli K-12 "cryptic" or deficient in 5'-nucleotidase (uridine diphosphate-sugar hydrolase) and 3'-nucleotidase (cyclic phosphodiesterase) activity. J.Bacteriol. 116:957-964
- Beacham, I.R., S. Garrett 1980. Isolation of Escherichia coli mutants (cpdB) deficient in periplasmic 2':3'-cyclic phosphodiesterase and genetic mapping of the cpdB locus. J.Gen.Microbiol. 119:31-34