CGSC Strain#: 3257
Strain Designation: AB3257     
Source of Strain: A.J. Pittard
Sex: F-
Chromosomal Markers: Δ(gpt-proA)62,
lacY1,
tsx-358,
glnX44(AS)
,
aroG365,
galK2(Oc)
,
aroH367,
hisG4(Oc)
,
rfbC1,
aroF363,
rpsL712(strR)
,
xylA5,
mtl-1,
ilvC7,
argE3(Oc)
,
thiE1,
tfr-3Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Wallace, BJ, J Pittard 1969. Regulation of 3-deoxy-D-arabino-heptulosonic 7-phosphate acid synthetase activity in relation to the synthesis of the aromatic vitamins in Escherichia coli K-12. J. Bacteriol. 99(3):707-12.