CGSC Strain#: 3303
Strain Designation: AB3303     
Source of Strain: A.J. Pittard
Sex: F-
Chromosomal Markers: lacY1,
glnX44(AS)
,
galK2(Oc)
,
pabB3,
hisG4(Oc)
,
rpsL700(strR)
or rpsL704(strR)
,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1,
tsx-29 or tsx-358Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Huang, M, J Pittard 1967. Genetic analysis of mutant strains of Escherichia coli requiring p-aminobenzoic acid for growth. J. Bacteriol. 93(6):1938-42.