CGSC Strain#: 3441
Strain Designation: AB3441     
Source of Strain: J. Stadler
Sex: F'
Episome/Plasmid: F8
Plasmid Markers/Mutations: Chromosomal Markers: araC14,
lacY1,
tsx-57,
glnX44(AS)
,
galK2(Oc)
,
metC56,
xyl-7 or xylA5,
lldD1,
thiE1,
tfr-5Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: - Stadler, J, EA Adelberg 1972. Temperature dependence of sex-factor maintenance in Escherichia coli K-12. J. Bacteriol. 109(1):447-9.
- Korner, A, BB Magee, B Liska, KB Low, EA Adelberg, D Soll 1974. Isolation and partial characterization of a temperature-sensitive Escherichia coli mutant with altered glutaminyl-transfer ribonucleic acid synthetase. J. Bacteriol. 120(1):154-8.