CGSC Strain#: 4262
Strain Designation: KL726     
Source of Strain: M.C. Cappello
Other Designations: KLF8/MA50
Sex: F'
Episome/Plasmid: F108
Plasmid Markers/Mutations: Chromosomal Markers: thr-1,
leuB6(Am)
,
fhuA2::IS2,
lacY1,
glnX44(AS)
,
gal-6,
λ-,
cys-46,
lysA24,
malT1(λ
R)
,
xyl-7,
mtlA2,
thiE1Strain Comments: - This strain must be grown on medium containing thi, thr, leu and lacking cys and lys. F108 probably carries the relA1 mutation.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- ::IS2 = Insertion of IS2
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
References: - Low, KB 1972. Escherichia coli K-12 F-prime factors, old and new. 36(4):587-607.