CGSC Strain#: 4276
Strain Designation: W1485E     
Source of Strain: C. Yanofsky
Sex: F+
Chromosomal Markers: glnV42(AS)
,
λ-,
rpoS396(Am)
,
rph-1Strain Comments: - Strain W1485 is E. coli K-12 wild-type made LAM- by UV. This derivative carries a suppressor, which W1485 does not have. We have designated it W1485E, to distinguish it from the parent strain.
- The reference below describes W1485.
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- glnV42(AS) was formerly called supE42
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
References: - Lederberg, J., E.M. Lederberg 1953. Genetic studies of lysogenicity in Escherichia coli. Genetics 38:51-64