CGSC Strain#: 4282
Strain Designation: KL737      Source of Strain: M.C. Cappello
Other Designations: F254/KL251
Other Designations: OR-F4/KL251
Sex: F' Episome/Plasmid: F254
   Plasmid Markers/Mutations: lac , hemB , phoA , proC , purE , rna , lipA , lipB
Chromosomal Markers: araC14, leuB6(Am), secA206(aziR), fhuA23, lacZ36, proC32, tsx-67, purE42, glnX44(AS), λ^-, trpE38, recA1, rpsL109(strR), xylA5, mtl-1, metE70, thiE1
Strain Comments:

• PO 59 is sometimes called OR11, and the strain of origin is sometimes called Hfr OR11.
• This strain should be maintained on medium containing thi, met, tryp, leu and lacking ade, pro.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• purE42-- :Strains with this marker are derivatives of Χ148 and may carry malA38LAMR and a suppressor of the Mal^- phenotype, sup-78,near his.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• proC32 was formerly called proC65
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• aziR = azide resistance
• strR = streptomycin resist.

References:

• Berg, CM, R Curtiss 1967. Transposition derivatives of an Hfr strain of Escherichia coli K-12. Genetics 56(3):503-25.