CGSC Strain#: 4521
Strain Designation: AT2681      Source of Strain: A.L. Taylor
Sex: F- Chromosomal Markers: lacY1 or lacZ4, glnX44(AS), λ^-, hisG1, purF1, glyA6, rpsL8 or rplL9(L?) or rpsL14, malT1(λ^R), xyl-7, mtlA2, ΔargH1, thiE1
Strain Comments:

• We have reports that this strain is temperature-sensitive and requires nicotinate for growth at 42 degrees C on minimal medium. It is also said to be P1^R.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• purF1-- is a very stable mutation and may be an intragenic deletion.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant
• strR = streptomycin resist.

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