CGSC Strain#: 4612
Strain Designation: WH-1      Source of Strain: Hoffman & Wilhelm
Sex: F' Episome/Plasmid: F196
   Plasmid Markers/Mutations: serU132(AS)
Chromosomal Markers: phoA4(Am), trp-37, his-45, recA1, rpsL144(strR), valR116
Strain Comments:

• phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• valR116-- the nature of Val^R is not known.
• Am = amber(UAG) mutation
• strR = streptomycin resist.
• AS = amber(UAG) suppressor

References:

• Hoffman, E.P., R. Wilhelm 1970. Genetic mapping and dominance of the amber suppressor, Su1 (supD) in Escherichia coli K-12. J.Bacteriol. 103:32-36