CGSC Strain#: 4617
Strain Designation: S26R1d      Source of Strain: A. Garen
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22, phoA4(Am), glnV33(AS), ompF627(T2R), fadL701(T2R), relA1, pitA10, spoT1, rrnB-2, mcrB1, creC510
Strain Comments:

• phoA4(Am)-- is Garen's phoAS26 . It is suppressed by leuX151, glnV33(=supE33) and serU132(=supD32).
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• glnV33(AS) was formerly called supE33
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Const = constitutive
• T2R = T2 Resistant

References:

• Garen, A., O. Siddiqi 1962. Suppression of mutations in the alkaline phosphatase structural cistron of E. coli. Proc.Natl.Acad.Sci.USA 48:1121-1127
• Garen, A., S. Garen, R. Wilhelm 1965. Suppressor genes for nonsense mutations. I. the Su-1, Su-2, and Su-3 genes of Escherichia coli. J.Mol.Biol. 14:167-187