CGSC Strain#: 5395
Strain Designation: ED1032      Source of Strain: P. Broda et al.
Sex: Hfr    Point of Origin#: 201(TP3)   Map Position: 58.00   Direction of Marker Transfer: Clockwise
Chromosomal Markers: Δ(gpt-lac)5, glnX44(AS), λ^-, TP3, relA1, rpsE2123(SpcR), thiE1
Strain Comments:

• Δ(gpt-lac)5-- is the deletion Δ( pro-lac)_X111 of Jacob and originated in the strain Hfr 3000 X111.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• TP3-- is F42-114 inserted between his (44) and thyA (68.8) his lacI-A > thyA
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• SpcR = spectinomycin resistant

References:

• Broda, P, P Meacock, M Achtman 1972. Early transfer of genes determining transfer functions by some Hfr strains in Escherichia coli K12. Mol. Gen. Genet. 116(4):336-47.