CGSC Strain#: 5396
Strain Designation: ED1039     
Source of Strain: P. Broda
Sex: Hfr
Point of Origin#: 125(TP4)(ts)
Map Position: 43.00
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: Δ(gpt-lac)5,
glnX44(AS)
,
λ-,
TP(lacA-lacI)4,
relA1,
rpsE2123(SpcR)
,
thiE1Strain Comments: - Δ(gpt-lac)5-- is the deletion Δ( pro-lac)X111 of Jacob and originated in the strain Hfr 3000 X111.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- TP(lacA-lacI)4-- is a transposition of lac due to the integration of F42-114 (FTS114- lac+ between his and trp. trp--uvrC--> lacA-I-- his
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- SpcR = spectinomycin resistant
References: - Masters, M, P Broda 1971. Evidence for the bidirectional replications of the Escherichia coli chromosome. Nature New Biol. 232(31):137-40.
- Broda, P, P Meacock, M Achtman 1972. Early transfer of genes determining transfer functions by some Hfr strains in Escherichia coli K12. Mol. Gen. Genet. 116(4):336-47.