CGSC Strain#: 4829
Strain Designation: E15     
Source of Strain: A. Garen
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10,
fhuA22,
ΔphoA8,
ompF627(T2R)
,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
rrnB-2,
mcrB1,
rob-1,
creC510Strain Comments: - spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Const = constitutive
- T2R = T2 Resistant
References: - Hayashi, H., J.P. Koch, E.C.C. Lin 1964. Active transport of L-a-glycerophosphate in Escherichia coli. J.Biol.Chem. 239:3098-3105
- Koch, J.P., H. Hayashi, E.C.C. Lin 1964. The control of dissimilation of glycerol and L-alpha-glycerophosphate in Escherichi coli. J.Biol.Chem. 239:3106-3108
- Sarthy, A., S. Michaelis, J.R. Beckwith 1981. Deletion map of the Escherichia coli structural gene for alkaline phosphatase, phoA. J.Bacteriol. 145:288-292