CGSC Strain#: 4969
Strain Designation: PL8-31     
Source of Strain: R.C. Greene
Other Designations: EWH154
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
Δ(gpt-proA)62,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
λ-,
Rac-0,
hisC3,
rfbC1,
metG87,
serA25,
metK86,
Δ(speC-glcB)63,
rpsL25(strR)
,
kdgK51,
xylA5,
mtl-1,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Greene, R.C., J.V. Hunter, E.H. Coch 1973. Properties of metK mutants of Escherichia coli K-12. J.Bacteriol. 115:57-67