CGSC Strain#: 4953
Strain Designation: H514      Source of Strain: H. Hoffmann-Berling
Sex: F- Chromosomal Markers: thyA26, thyA46, endA2, uvrA103, arg-48, φX^s
Strain Comments:

• This K-12 x C hybrid is devoid of K-12 host modifying and restricting properties.
• thyA26-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• thyA46-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• φX^s-- : φX sensitivity is derived from E. coli C
• = good miniprep dsDNA quality

References:

• Vosberg, HP, H Hoffmann-Berling 1971. DNA synthesis in nucleotide-permeable Escherichia coli cells. I. Preparation and properties of ether-treated cells. J. Mol. Biol. 58(3):739-53.