CGSC Strain#: 4947
Strain Designation: Q13      Source of Strain: A.M. Reiner
Sex: Hfr    Point of Origin#: 21   Map Position: 97.00   Direction of Marker Transfer: Clockwise
Chromosomal Markers: rna-19, gdhA2, his-95, tyrA6, relA1, pnp-13, spoT1, metB1
Strain Comments:

• For description of other possible defects in this strain, see references.
• rna-19-- : Also References \\ Weatherford et al. 1972 J.Biol.Chem.247:540 \\ Studier 1975 J.Bacteriol.124:307 \\ Apirion and Watson 1975 J.Bacteriol.124:317
• rna-19-- : Additional references to strain A19 and its derivatives: \\ Gesteland 1966 J.Mol.Biol.16:67 \\ Reiner 1969 J.Bacteriol. 97:1431,1437,1522
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rna-19 was formerly called rns-19
• tyrA6 was formerly called tyr-451

References:

• Gesteland, RF 1966. Isolation and characterization of ribonuclease I mutants of Escherichia coli. J. Mol. Biol. 16(1):67-84.
• Reiner, A.M. 1969. Isolation and mapping of polynucleotide phosphorylase mutants of Escherichia coli. J.Bacteriol. 97:1431-1436
• Reiner, AM 1969. Characterization of polynucleotide phosphorylase mutants of Escherichia coli. J. Bacteriol. 97(3):1437-43.
• Reiner, AM 1969. Genetic locus for ribonuclease I in Escherichia coli. J. Bacteriol. 97(3):1522-3.
• Weatherford, SC, L Rosen, L Gorelic, D Apirion 1972. Escherichia coli strains with thermolabile ribonuclease II activity. J. Biol. Chem. 247(17):5404-8.
• Apirion, D., N. Watson 1975. Mapping and characterization of a mutation in Escherichia coli that reduces the level of ribonuclease III specific for double-stranded ribonucleic acid. J.Bacteriol. 124:317-324
• Studier, FW 1975. Genetic mapping of a mutation that causes ribonucleases III deficiency in Escherichia coli. J. Bacteriol. 124(1):307-16.