CGSC Strain#: 5349
Strain Designation: MX383      Source of Strain: M.P. Oeschger
Sex: F' Episome/Plasmid: F196
   Plasmid Markers/Mutations: serU132(AS)
Chromosomal Markers: lacZ53(Am), λ^-, trpA49(Am), his-90, gyrA20(NalR), recA1, relA1, arg-47
Strain Comments:

• The strain is Val^R ( valR118).
• This strain must be grown on medium containing histidine and lacking arginine and tryptophan.
• lacZ53(Am)-- is the Paris lac^-_{Y14 amber}.
• trpA49(Am)-- is the amber mutation formerly called trp^-_8am by Brenner.
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• arg-47-- is a point mutation in the argC-H cluster
• Am = amber(UAG) mutation
• NalR = Nalidix.acidResist.
• AS = amber(UAG) suppressor

References:

• Oeschger, MP, SL Woods 1976. A temperature-sensitive suppressor enabling the manipulation of the level of individual proteins in intact cells. Cell 7(2):205-12.