CGSC Strain#: 12264
Strain Designation: TA516      Source of Strain: C.L. Squires
Sex: F- Episome/Plasmid: pTRNA65
   Plasmid Markers/Mutations: ori_p15A, aadA(strR,SpcR), trpT , thrV , ileU , aspT , alaU , gltT
Chromosomal Markers: ara-600, Δ(rrsH-rrlH)770, Δ(codB-lacI)3, Δ(rrlG-rrsG)772::lacZ, Δ(rrlD-rrsD)773::cat, Δ(rrsA-rrlA)771, Δ(rrsB-rrlB)769, thiE1, Δ(purD-metA)768
Strain Comments:

• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• Δ(purD-metA)768 was formerly called Δ(purDH-rrnE-metA)
• Δ(rrlD-rrsD)773::cat was formerly called Δ(rrsD-ileU-alaU-rrlD)25::cat
• Δ(rrlG-rrsG)772::lacZ was formerly called Δ(rrsG-gltW-rrlG)30::lacZ
• Δ(rrsA-rrlA)771 was formerly called Δ(rrsA-ileT-alaT-rrlA)34
• Δ(rrsB-rrlB)769 was formerly called Δ(rrsB-gltT-rrlB)101
• Δ(rrsH-rrlH)770 was formerly called Δ(rrsH-ileV-alaV-rrlH)103
• thiE1 was formerly called thi-1
• = chloramphenicol resistant
• SpcR = spectinomycin resistant
• strR = streptomycin resist.

References:

• Asai, T, D Zaporojets, C Squires, CL Squires 1999. An Escherichia coli strain with all chromosomal rRNA operons inactivated: complete exchange of rRNA genes between bacteria. Proc. Natl. Acad. Sci. U.S.A. 96(5):1971-6.
• Asai, T, C Condon, J Voulgaris, D Zaporojets, B Shen, M Al-Omar, C Squires, CL Squires 1999. Construction and initial characterization of Escherichia coli strains with few or no intact chromosomal rRNA operons. J. Bacteriol. 181(12):3803-9.