CGSC Strain#: 5964
Strain Designation: DF456      Source of Strain: D. Fraenkel
Sex: F- Chromosomal Markers: fhuA2::IS2, lacY1, tsx-6, glnX44(AS), gal-6, λ^-, gatC49, gatA50, srlC?-49, recA1, argG6, rpsL104, xyl-7, mtlA2, pfkA300::Mu, metB1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• xyl-7 was formerly called xylA7
• = strep-resistant
• ::IS2 = Insertion of IS2
• AS = amber(UAG) suppressor

References:

• Thomson, J, PD Gerstenberger, DE Goldberg, E Gociar, A Orozco de Silva, DG Fraenkel 1979. ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt. J. Bacteriol. 137(1):502-6.