CGSC Strain#: 5705
Strain Designation: S730      Source of Strain: A.H. Stouthamer
Sex: F- Chromosomal Markers: fhuA2::IS2, lacY1, tsx-70, glnX44(AS), gal-6, λ^-, purB51, hemA30, trpC45, pabB5, his-68, rfbC1, tyrA2, galP63, rpsL125(strR), malT1(λ^R), xyl-7, mtlA2, thiE1, pyr-65
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hemA30-- : hemA mutant strains must be supplemented with Δ-aminolevulinic acid, e.g., L + 10-50 ug/ml ΔALA
• pyr-65-- The locus of this mutation is not known.
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• ::IS2 = Insertion of IS2
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant
• strR = streptomycin resist.

References:

• Guest, J.R. 1969. Biochemical and genetic studies with nitrate reductase C-gene mutants of Escherichia coli. Mol.Gen.Genet. 105:285-297
• Semple, KS, DF Silbert 1975. Mapping of the fabD locus for fatty acid biosynthesis in Escherichia coli. J. Bacteriol. 121(3):1036-46.