CGSC Strain#: 12320
Strain Designation: RRL150     
Source of Strain: D.J. Sherratt
Sex: F-
Chromosomal Markers: thr-1,
araC14,
ΔleuB261::lacZp-lacI- mCherry,
cat,
Δ(gpt-proA)62,
lacY1,
tsx-33,
qsr'-0,
glnX44(AS)
,
ΔgalK539::lacZp-tetR-mCerulean,
kan,
λ-,
Rac-0,
hisG4(Oc)
,
rfbC1,
mgl-51,
rpoS396(Am)
,
rpsL31(strR)
,
kdgK51,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1Strain Comments: - ΔleuB261::lacZp-lacI- mCherry,cat-- This allele has a lacZp promoter driving expression of a lacI-mCherry fusion protein. This is followed by a chloramphenicol resistance cassette.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- ΔgalK539::lacZp-tetR-mCerulean,kan-- This allele has a lacZp promoter driving expression of a tetR-mCerulean fusion protein. This is followed by a kanamycin resistance cassette.
- Δ(gpt-proA)62 was formerly called proA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- = kanamycin resistant
- = chloramphenicol resistant
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Reyes-Lamothe, R, C Possoz, O Danilova, DJ Sherratt 2008. Independent positioning and action of Escherichia coli replisomes in live cells. Cell 133(1):90-102.