CGSC Strain#: 5791
Strain Designation: E15, LAM-      Source of Strain: E. Meyerowitz
Other Designations: E15 LAM-
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, ΔphoA8, λ^-, ompF627(T2R), fadL701(T2R), relA1, pitA10, spoT1, rrnB-2, mcrB1, creC510
Strain Comments:

• This is a LAM- derivative of strain E15.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• T2R = T2 Resistant

References:

• Hayashi, H., J.P. Koch, E.C.C. Lin 1964. Active transport of L-a-glycerophosphate in Escherichia coli. J.Biol.Chem. 239:3098-3105