CGSC Strain#: 6455
Strain Designation: YYC100      Source of Strain: J.E. Cronan
Sex: F- Chromosomal Markers: araC14, leuB6(Am), secA214(aziR) or secA216, Δ(aroP-aceF)73, zac-284::Tn10, ts100, lacZ36, proC32, tsx-67, purE42, glnX44(AS), λ^-, trpE38, lysA23, xylA5, mtl-1, metE70, thiE1, fhuA23 or fhuA54
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• zac-284::Tn10-- cotransduces ca. 99% with aceEF; in YYC100, see comment about ts mutation, not present in YYC186.
• ts100-- : YYC100 has been reported to be temperature sensitive at 42C with the temperature sensitivity co-transduced with zac-284::Tn10. The zac-284::Tn10 strain YYC186 is not TS.
• purE42-- :Strains with this marker are derivatives of Χ148 and may carry malA38LAMR and a suppressor of the Mal^- phenotype, sup-78,near his.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• proC32 was formerly called proC65
• thiE1 was formerly called thi-1
• = tetracycline resistant
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

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