CGSC Strain#: 6439
Strain Designation: C8     
Source of Strain: A. Garen
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22,
phoR78,
fadL701(T2R)
,
relA1,
pitA10,
spoT1,
rrnB-2,
mcrB1,
creC510Strain Comments: - spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- rrnB-2-- : loss of spacer loop sequence
- creC510-- The constitutive phenotype is due to an R77P amino acid substitution
- creC510 was formerly called phoM510
- Const = constitutive
- T2R = T2 Resistant
References: - Echols, H., A. Garen, A. Torriani 1961. Genetic control of repression of alkaline phosphatase in E. coli. J.Mol.Biol. 3:425-438
- Kreuzer, K.N., C. Pratt, A. Torriani 1975. Genetic analysis of regulatory mutants of alkaline phosphatase of Escherichia coli. Genetics 81:459-468