CGSC Strain#: 6448
Strain Designation: χ2469      Source of Strain: Maturin & Curtiss
Other Designations: chi2469
Sex: F- Chromosomal Markers: Δ(proB-lac)41, glnV42(AS), λ^-, pyrF11, cdd-7, pyrG77, thyA702, dnaB43(ts), cycA1, deoC8, T3R
Strain Comments:

• thyA702-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• glnV42(AS) was formerly called supE42
• AS = amber(UAG) suppressor
• ts = Temperature Sensitive

References:

• Maturin, LJ, R Curtiss 1981. Role of ribonucleic acid synthesis in conjugational transfer of chromosomal and plasmid deoxyribonucleic acids. J. Bacteriol. 146(2):552-63.